Thanks 23. For double label immunostaining and colocalization of two proteins in
ID: 198939 • Letter: T
Question
Thanks 23. For double label immunostaining and colocalization of two proteins in cells you would need A. 2 primary antibodies that can recognize the same antigen B. 2 primary antibodies produced in different animals C. 2 secondary antibodies with similar fluorophores D. 2 secondary antibodies that recognize the same species of primary antibodies E. All of the above 24. Mutations or breaks in genomic DNA can be repaired in different ways. Which way is the most prone to cause nm A. Non homologous end joining. B. Excision repair. C. Homologous recombination D. Mismatch repair. 25. This type of posttranslational modification for proteins can increase the likelihood of degradation A. Phosphorylation B. Ubiquitination C. Lipidation D. Glycosylation If you don't have an antibody to your protein and want to visualize your protein in cells by fluorescent microscopy you could... A. Perform in situ hybridization for your protein B. Translate your protein with florescent dyes on the ribosome C. Fuse the cDNA of your protein to that of GFP and introduce into the cells D. All of the above. E. None of the above. 27. Watson and Crick presented the first correct model for the structure of DNA. What method was used to get their data? A. Nuclear Magnetic Resonance B. Chemical crosslinking C. Circular Dichroism D. X-ray diffraction.Explanation / Answer
As the double label immunostaining name suggest that two different primary and secondary antibodies are used. Here E is the correct answer. Please check the link: http://www.ihcworld.com/_protocols/general_IHC/immunofl_double_squential.htm
Non homologous end joining is most prone because in repair is due to non homologous chromosome and that lead to unwanted events in cell division.
Ubiquitinaition direct the degradation of protein.
If GFP protein will fused at the downstream to protein of interest than it will expressed with you desired protein and can be seen in microscope.
Watson and crick obtained DNA model by X-ray diffraction.
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