13. Given a log phase bacterial culture with 1 x 10s cells per ml and a generati

Question

13. Given a log phase bacterial culture with 1 x 10s cells per ml and a generation time of 30 minutes, how long does it take the culture to reach a density of 6.4 x 107 cells per ml? A. 1 hour B. 2 hours C. 3 hours D. 4 hours E. 6 hours 14. Which of the following is NOT a possible reason for entry into the stationary phase of growth? A. Nutrient limitation B. Limited oxygen availability C. Toxic waste accumulation D. Critical population density reached E. All of the above are possible reasons are associated with certain chronic infections and do not respond to 15. antibiotic treatment. A. Bacteria B. Daughter cells C. Pathogens D. Persisters E. None of the above

Explanation / Answer

Question number 13. - 3 hours.

Explanation - As bacteria divide by binary fission so one parent cell form two daughters cell, again two daughters cells act as parent cell. So The number of parents become double after each division. The generation time is the time in which a complete cycle of division occur. So it takes 6 division means 3 hours to reach the population.

Question number 14 - All of the above are possible reasons.

All the reasons are either results into competition or kill the bacteria so that growth rate occur equal to death rate so responsible for entry into stationary phase.

Question number 15- Persisters is the answer.

These are some bacterial cell without genetic modification which persist against antibiotics.

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