2. (+pts) Describe what is happening in the following scenario to the repressor,

Question

2. (+pts) Describe what is happening in the following scenario to the repressor, operator, cAMP production, CAP, CAP binding site, RNA polymerase, and whether or not mRNA gets trans sure to state what effects Lactose and Glucose are having in the appropriate space E coli with the genotype, L:OZ:/Fo. Low levels oflactose and low levels of glucose. Lac operon tOzlasUrepressor gene is normal, mutation in the operator prevents represso from binding to the operator, 8-galactosidase gene is normal Lac I can be expressed as L. Operator can be expressed as 0. Lac Z can be expressed as Z. Etcetera. lacl lacZ lacY lacA CAP P Repressor &Operator; (1 pts): cAMP production and CAP (1 pts): CAP binding site & RNA polymerase (1 pts): mRNA (1 pts): words: 55 of 204 100% Print Layout View Sec 1 Pages: 1 or 2

Explanation / Answer

Given genotype: I+OcZ+

Given condition: No lactose and no glucose.

Since operator sequence is mutated such that the repressor protein can no longer bind to it. So, the operon is constitutively active irrespective of the presence or absence of lactose.

When lactose is absent, the repressor is functional. But, it can not bind to the operator.

Since the operator is not bound by the repressor, RNA polymerase can bind to the promoter.

Since RNA polymerase can bind to the promoter, transcription can take place. So, mRNA will be produced.

When glucose is absent, cAMP-CAP complex can bind to the upstream region and recruit RNA pol to the promoter.

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