help with batch production calculations and how to solve them please? The follow
ID: 198431 • Letter: H
Question
help with batch production calculations and how to solve them please?
The following are examples of Batch Production Record calculations. Perform the calculations based on the provided values, use scientific notation where necessary and round to the appropriate number of significant digits. Ignore fields with N/A. 23. Begin Stain: N/A End Stain: NIA | Operator Initials/Date Verifier Initials/Date Count 1 Chamber 1 Chamber 2 Chamber 3 Chamber 4 Avg. Viable Cells/mL 3.61e7 3.26e7 3.24e7 3.19e7 | Total Cells/mL 3.71e7 3.37e7 3.39e7 3.35e7 Viability (%) 97.4 96.8 95.8 95.2 N/A NIA Percent Difference (Repeat all counts if Percent Difference is > 20.0%) High Viable Low Viable High Viable + PercentPercent Verifier Initials/Date Operator DifferenceDifference |-| Cells/mLCells/mLx (%) Initials/Date -1 20.0% a Yes o No Viable 200 N/A N/A 24 Stimulation Ratio B Cells Required for irradiation Total T Cells B Cells T Ceils 5 2.47e6 RCatle petatttenaTarget B Cell IrradiationVolume of B Cell to B Cells Required for Irradiation Concentration Resuspended 2.00E6 (mL)Explanation / Answer
(23)
High viable cells/ml - low viable cells/ml x 200 % + (high viable + low viable) = percent difference
(3.61-3.19) * 200% + (3.61+3.19)
0.42 * 200% + 6.80= 7.64 %
Percent difference = 7.64 %, So the percent difference is less than 20 %.
(24)
B-cells required for irradiation = T-cells + B-cells * total T cells
= 5 + 1 *2.47 =14.82
Volume of B-cell to re-suspended = B-cells required for irradiation + Target B-cell radiation concentration
= 14.82 + 2 =16.82 ml
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