. N-tosyl-L-phenylalanine chloromethylketone (TPCK) specifically labels 1sa i of
ID: 198219 • Letter: #
Question
. N-tosyl-L-phenylalanine chloromethylketone (TPCK) specifically labels 1sa i of chymotrypsin. How would you modify the structure of this inhibitor to design an inhibitor for elastase? 2. Explain the biochemical rationale behind trypsin's ability to activate trypsinogen by autocatalysis. (i.e. how does trypsin do this?) 3. Explain how the three amino acids, His57, Ser195, and Asp102, so distantly separated in the sequence of an enzyme can catalyze a reaction occuring in the space of a few tenths of a nanometer. (Hint: keep in mind things that you learned for the midterm) 4. Discuss the importance of substrate analogs for studying enzyme mechanisms.Explanation / Answer
The activation of trypsinogen to trypsin occurs in the small intestine which is either initiated by centerokinase or, alternatively or through autocatalytic process which is catalysed by trypsin. It is done through active site titrant for trypsin based on the acyl enzyme.
Substrate analogs have chemical structure which resemble the substrate molecule, mostly seen in an enzyme-catalyzed chemical reaction. They act as competitive inhibitors of an enzymatic reaction.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.