NAME BIOL 200 Mastering Laboratory Math 1. We have a 1.5 M solution of Tris-HCL.
ID: 198086 • Letter: N
Question
NAME BIOL 200 Mastering Laboratory Math 1. We have a 1.5 M solution of Tris-HCL. We want 20 ml of 0.5 M solution. a) What is the starting concentration (C1)? b) What is the final concentration (C2) c) What is the starting volume (V1)? d) What is the final volume (V2)? e) Write out the equation used to solve this HC Solve the equation g) If you had to make this solution in a 50 mL. conical tube. How much diluent would you add? h) This is an aqueous solution. What was your diluent? we have 10% SDs. we need 10 mL of a 2% solution. a) What is the starting concentration? b) What is the final concentration? c) What is the starting volume? d) What is the final volume? e) Write out the equation used to solve this 2, Solve the equationExplanation / Answer
Ans. 1.a. C1 = 1.5 M
#b. C2 = 0.5 M
#c. V1 = ? (to be determined)
#d. V2 = 20.0 mL
#e. C1V1 (starting solution) = C2V2 (Final solution)
#f. V1 = C2V2 / C1 = (0.5 M x 20.0 mL) / 1.5 M = 6.67 mL
#g. Volume of diluent = Vol of final solution (V2) – Required vol. of staring soln.
= 20.0 mL – 6.67 mL
= 13.33 mL
#h. For aqueous solutions, water is used as diluent.
#2. C1 = 10%
#b. C2 = 2 %
#c. V1 = ? (to be determined)
#d. V2 = 10.0 mL
#e. C1V1 (starting solution) = C2V2 (Final solution)
#f. V1 = C2V2 / C1 = (2 % x 10.0 mL) / 10 % = 2 mL
#g. Volume of diluent = Vol of final solution (V2) – Required vol. of staring soln.
= 10.0 mL – 2.0 mL
= 8.0 mL
#h. For aqueous solutions, water is used as diluent.
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