11) The fatty acid contents (%) were determined using gas chromatography. Approx
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Question
11) The fatty acid contents (%) were determined using gas chromatography. Approximately 0.1g of SAMPLE (0.1017g for broccoli sample) was weighed into 13x100mm test tube and 2mL of methanolic HCl was added. The samples were heated at 50°C for 2 hours to complete the methylation of fatty acids create fatty acid methyl esters (FAMES). Following this 0.100mL of internal standard (3.03mg methyl C17:0/mL of hexane) was added and mixed well. A portion of this was injected into a gas chromatograph for fatty acid analysis. The resultant chromatograms are presented separately. Use this information to answer the following questions.
a) Calculate the amount of internal standard (mg C17:0/test tube) added to the test tube with the SAMPLE FAMES. What is the area for C17:0 in the broccoli SAMPLE Chromatogram?
System OC4 Method Falty Acids 20m User Kelwin Lien Acquired 03020014 1109 04 AM Processed 06402/2014 12 12 53 PM Pinted 06/0212014 12 46 43 PM Index! Time! Heght! Areal Area%| Name 3 3 8 8 40 60 s0 100 12I 140 14 1 16 0 , 170 17.1 18 1 82 83 WO 20.1 204 220 821 723 22 sExplanation / Answer
a) .303 mg /ml of internal standard C17:0/ tube added in sample FAME means fatty acid methyl ester. 2134.5 uV.min is the area of C17:0 in broccoli sample.
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