4) The DNA sequence of a short gene from a sea slug, and the mature RNA synthesi

Question

4) The DNA sequence of a short gene from a sea slug, and the mature RNA synthesized from this gene, are shown below DNA sequence: A -T mature mRNA: 5' - cap-AUCUÇAUGUGrGAACGCUGACUAGAAAAAAAAAA- 3 a. In the DNA sequence, draw boxes around two exons in the gene. The splicing machinery does NOT pay attention to codons. The splicing of this DNA demonstrates that. How? 2pts b. Draw boxes around any structures or bases that were added to the RNA during processing. (1pts) c. How many amino acids are in the peptide encoded by the gene?

Explanation / Answer

Exon 1 : 5'-( AGCATCTCATGTGCGA)-3'

Exon 2: 5'-(ACGCTGACTAGC)-3'

Intron : 5' - (GTCCTG)-3'

If it follow general splicing rule then intron sequence be like 5'- ( GU -----------A -------AG)-3'

The last part containing ( AAAAAAAAAA -3') were added to RNA during processing. ( Poly A tail).

There are 7 amino acids in the peptide

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