_ (d) His (4) Amide group-containing amino acid -(e) Cys (5) C, is not chiral 5.

Question

_ (d) His (4) Amide group-containing amino acid -(e) Cys (5) C, is not chiral 5. (10 pts) You want to determine the amino acid sequence of a peptide. Answer the following questions (a) When the peptide was digested by trypsin (which cuts the carboxy) side of L.ys and Arg). thre fragments were method, were as follows: produced. The sequences of the three purified fragments, determined by the Edman Thr-Phe-Gly-Arg Glu-Met-Val-Lys Ala-Arg The overall sequence is the combination of these three fragments. Based on this information alone, how many possible overall sequences are there for this peptide? (b) When this peptide was digested by CNBr (which cuts the carboxyl side of e), two fragments wer obtained with the following sequences: Val-Lys-Thr-Phe-Gly-Arg Ala-Arg-Glu-Met Using the overlap peptides, determine the entire amino acid sequence of this peptide

Explanation / Answer

ANSWER 1) the 2 possible combinations are a) 1-2-3 or b)2-1-3 (1,2,3 being the sequences mentioned above in sequential manner)

ANSWER 2) the complete sequence would be ala-arg-glu-met-val-lys-thr-phe-gly-arg

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