How would the student go about determining if the protein powder contains the nu

Question

How would the student go about determining if the protein powder contains the number of grams of protein that it advertises? OA. Dilute the protein powder with water at various concentrations and measure absorbance with a spectrophotometer. B. Add Biuret reagent to a sample of protein powder and look for the presence/absence of color change. O C. Use a standard curve obtained by diluting whey protein into various concentrations, adding biuret reagent and measuring absorbance with a spectrophotometer D. This experiment is not possible.

Explanation / Answer

the answer is "B".

biuret test involves an alkaline mixture of copper sulphate and potassium hydroxide.the normal colour of the biuret reagent is blue, but when it gets contact with peptide bond(protein), the colour of the reagent turns violet.the amount of protein can be known by taking a spectroscopic reading at a wavelength of 540 nm.

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