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1) Give a possible genotype lac, lacP /acO lacZ lacY) of a lac operon that produ

ID: 197044 • Letter: 1

Question

1) Give a possible genotype lac, lacP /acO lacZ lacY) of a lac operon that produces - galactosidase and permease results under the following conditions. Genotypes are designated by a superscript symbol. Functional genes are represented by a + and defective genes are designated by a". For example, functional lacl is written, laci* and nonfunctional is written lact. For the enzymes, a plus sign (+) indicates that the enzyme is synthesized and a minus sign (-) indicates that the enzyme is not synthesized. (4 pts) Lactose absent Lactose present -Gal Perm B-Gal Perm a. b. C. d. a, C.

Explanation / Answer

a. lacI+ lacP+ lacO+ lacZ+ lacY+

b. lacI- lacP+ lacO+ lacZ+ lacY+
or
lacI+ lacP+ lacO- lacZ+ lacY+

c. lacI- lacP+ lacO+ lacZ- lacY+
or
lacI+ lacP+ lacO- lacZ- lacY+
or
lacI- lacP+ lacO- lacZ- lacY+

d. lacI+ lacP- lacO+ lacZ+ lacY+
or
lacI+ lacP+ lacO+ lacZ- lacY-
or
lacI+ lacP- lacO+ lacZ- lacY-