Oxidation- Fermentation Medium (observation & interpretation) PH indicator .Brom
ID: 197002 • Letter: O
Question
Oxidation- Fermentation Medium (observation & interpretation) PH indicator .Bromthymol Blue E. coli P aeraginosa A faecalis 1.Some microbiologist recommend a pair of 0-F basal media [without carbohydrate) along with the carbohydrate media. Why do you think this is done? 2.All enterics are facultative anaerobes. That is they have both respiratory and fermentative enzymes. What color would you expect for organisms in O-F media inoculated a wih an enteric? 3. Sup[pose that you examined your tubes after incubating, you noticed that the unsealed control contained slight yellowing at the top. Suppose further that pair #1 showed complete yellowing of both tubes and pair #2 and #3 showed slightly yellowing of the unsealed tube. Assuming all other tubes were green, what conclusion could you safely make Which results if any, are reliable? Why? Which results, if any, are not reliable? Why not?Explanation / Answer
Basal media (without carbs) enables you to perceive what the organism does with medium without the nearness of the sugar. Enables you to have an "active" negative control. Enteric bacteria are facultative anaerobes, which means they are equipped for both aerobic and fermentative metabolism. What results would you hope to see in both sealed and unsealed OF tubes with such a bacterium? The technique calls for three organisms to be inoculated. The slight yellowing in the unsealed control tube proposes there may be a contaminant (aerobic) in the media. This would roll out the slight improvement in the sets 2 and 3 faulty and most likely the tests ought to be done once more. Match 1 demonstrates that the organism is fermentative and conceivably oxidative, because of the total yellowing of the two tubes. We found that the oxidative just organisms tended to indicate substantially more than "slight" yellowing, so having the control recommends that sets 2 and 3 are negative, from our experience.
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