3C a. Match the following cell surface receptors to their cell type. Note that s

Question

3C a. Match the following cell surface receptors to their cell type. Note that some receptors may be expressed on more than one cell type. 10.5 point each, 2.5 points total) CD19, CD3, CD4, CD8, DXS, CD11b, cDiic, CD14, MHCL IMHCILgM Cell Type Cell Surface Receptors on this Cell Type CD4, CD8 Natural Killer Cells Dendritic Cells 3Cb. Fluorescence-activated cell sorting (FACS) utilizes their frequencies within a mixed cell population. FACS data is often displayed as "dot plots. You stain a sample of peripheral blood from a healthy mouse. Draw a dot plot that corresponds to a sample containing the celtypes indicated at the top of the plot. (4 pts) antibodies to identify cell types and T L Dendritic and NK cells 3D Write the name of a technique that uses antibodies that is best to complete the following situations. /4 pts total, 1 pt each) Situatiorn 1. You want to determine the level of IL-1 in the serum of your pet Which s best? 2. You need to know the proportion of neutrophils in the peripheral blood of a lab mouse 3. You want to determine if IkB is phosphorylated after TLR signaling 4. You want to know the specific location of CD4 T cells in a lymph node after immunization

Explanation / Answer

3 d)

1 - ELISA sandwich technique

2- Differential count or absolute counting of the neutrophills by flow cytometry or total count of white blood cells.

3) By stimulation with TLR ligands.

4) Using tetramer specific for CD4 population and CFSE staining by flow cytometry.

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