1. What is the difference between an F + and an Hfr bacterium? d. F + bacteria h

Question

1. What is the difference between an F+ and an Hfr bacterium?

d. F+ bacteria have a plasmid with only the essential genes for conjugation, while Hfr (high frequency recombinant) bacteria have an F plasmid that includes some nuclear genes.

2. Integration of linear transforming DNA into the chromosome

d. only occurs in laboratory-based systems in artificial competent cells.

3. What structure can the viral genome take?

4. The life cycle of lambda phage is controlled by accumulation of repressor proteins. For lysogeny to occur

a. F+ bacteria have a nonintegrated F plasmid, while Hfr bacteria have an F plasmid that is integrated into their main chromosome. b. F+ bacteria have an integrated F plasmid, while Hfr bacteria have a nonintegrated F plasmid. c. F+ bacteria have an F plasmid that includes some nuclear genes, while Hfr (high frequency recombinant) bacteria have an F plasmid with only the essential genes for conjugation.

d. F+ bacteria have a plasmid with only the essential genes for conjugation, while Hfr (high frequency recombinant) bacteria have an F plasmid that includes some nuclear genes.

2. Integration of linear transforming DNA into the chromosome

a. is not required for the expression for the transformed genes. b. is catalyzed by the RecA gene. c. almost never occurs because restriction endonuclease will degrade the DNA before it is integrated.

d. only occurs in laboratory-based systems in artificial competent cells.

3. What structure can the viral genome take?

a. ds-DNA b. ds-RNA c. ss-DN d. All of the listed responses are correct.

4. The life cycle of lambda phage is controlled by accumulation of repressor proteins. For lysogeny to occur

a. cI protein is repressed. b. cII protein is repressed. c. Cro protein predominates. d. cI protein predominates.

Explanation / Answer

Question 1.

Answer. (a)

Question 2.

Answer. (b)

Question 3.

Answer. (d)

Question 4.

Answer. (d)

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