Spectrophotometry Determination of Chlorophyll-a Concentration in Spinach Name L

Question

Spectrophotometry Determination of Chlorophyll-a Concentration in Spinach Name Lab Partner Section TA Name Score Questions 1. Why was it important to force the trendline in the plot of absorbance vs. concentration through the origin? Would adding the point (0, 0) to your data set have had the same effect? Why or why not? 2. Isopropanol is a good extraction solvent for plant pigments as it breaks down cell walls and is mis- cible with the pigments. Can you be sure that you only extracted chlorophyll-a from your spinach leaf? If not, what else might have been extracted?

Explanation / Answer

Because the absorbance at zero concentration must be zero. So it is forced.

Chlorophyll b may be present. Extraction should be made with 80% acetone

Chlorophyll is detected by absorbance spectrum. Maximum absorption seen at around 678 m/z

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