Alanine - Asparagine- Serine- Tryptophan -Glutamic acid- Arginine 2. (2 points)

Question

Alanine - Asparagine- Serine- Tryptophan -Glutamic acid- Arginine 2. (2 points) According to the conventional way of writing the sequence of a peptide or a protcin, which the C-terminal amino acid and which is the N-terminal amino acid in the above pentide (Oustion l is C-terminal: N-terminal: 3. (5 points total) Using the pK, values for lysine, determine the net charge at pl values of 1 ,4 and 12 (l pt cach) and calculate the isoelectric point (2 pts) pk. ofthe following groups: -COOH has 2.18 a-NH, has 895 R group has 10.53 Net charge at pll pH 12 pl 4. (6 points total) Name the following amino acids in three-Jetter code (2 pts each) c. Histidine COO CH2 H,N-C-H CH HC CH. CH H N K)

Explanation / Answer

2.

Thefirst amino acid is always present at the n-terminal region and The last amino acid is called as N terminal amino acid.

Here first amino acid of polypeptide chain is alanine and last amino acid is arginine.

So the free N terminal is of alanine.

The last amino acid is arginine and it have free C terminal.

3. At pH first = amino acid is positive charge

At pH 4 = amino acid is neutral charged.

at pH 12= amino acid is possitively charged.

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