Write a lab summary about lab Exercise 4 Culture Techniques. The Lab Summary sho

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Write a lab summary about lab Exercise 4 Culture Techniques. The Lab Summary should include the main concepts/techniques covered that day.The lab summary should be about one paragraph.

C @file:/ D:Downl 13pdf Lalboratory Exercise 4: Culture Techmiques ISS 2018) Objeerives: 1. Perfonn basic aseptie proccdures of microbiology 2. Understand some of the ditferent ways of culturing icroorganisms and viruses. 3. Have an appreciation fo the ubiquity and diversity of microorganisems Microorganisms are said to ubiquitous, that is, they seem be present everywhere-in the air we breath, the water we drink, the food we eat, on ur hody surfaces, and even inside our bodies The vast majority of these mioorganisms are harmless, bur care needs to taken in microbiology since some of them cause disease, especially if intreduced in large numbers Although individual micobial ells are microscopic. under the right conditions, they can quickly proliferate to form large populations that are ensily visible. Production of many cells causes a liquid medium to become cloudy or turbid. Altematively, a single, isclated cell on a solid surface may multiply until it forms a colony, which is a visible mound of millions of its descendants, all arising from one oell. [Fig 1] Both situations are used in microbiology. Pure cultures are often gnown in a liquid medium in a glass tub¢ with a spxial cap that allows air to enter. To protec a liquid culture, smanumber of cells (tbe ineculum) is added lo the sterile medium (containine necessary nutrients) and then incubated at a favorable temperarure. Fig 2 However, to first achieve a pure culture, it is usually pecessry to grua bacteria as solated eolonics on solid media. Solid mcdia can usually be prepared from liquid media simply by adding agar betore steriliztion. Upon ccoling, the agar Sos a solid gel. Petri plates are oommonly used vessels for solid madin. They provide a large surface nrea for ease in observing or retrieving many individual colenics, Different microorganisms often exhibit differences in colony morplology (shape), color, and sia, which also Ihelps distinguish them Fig 1]. The major challenge in getting isolated colonies on solid medium is to spread cut a few of the millicns of oiginal cells so that they are far apart: this almost always requires repeated stages of some dilution method. The streak plate is one metbod [see Fig. 3]; it involves drawing the microorpanisms hack and forrh across the surface of a solid medium with a wire loop. The process is repeated several times onto fresh areas of the plane usinga sterile loop every time [FigA] For a spread plate, a smallaoof Liquid coataining microorganisms is sprcad out over the surface of a plate with a bent glass rod, called a"Hockey Stick". If the cells were sufficiently diluted in the liquid, this allows them to fon separate colonies and thus be counted. In a pour plate, the diluted cells are mixed with molten agar while it is still warm (about 45 C), and poured into a petri plate. Solid media can also be used for goawing hacterial "Tawns" (confluent layers of oells) on which bacterial vinises (hacteriophage) can he cultivatad. Semi solid media are penerally used for testing the morility of bocteria, sinee the cells are able to pantraand .swint" through the soft tnodium. [Fig 5] .sb 4 sszu16,pdf Show sli | X O lype here to search

Explanation / Answer

So, in conclusion we can say that there are many techniques available to culture the bacteria. Bacteria is cultured on solid media. Aspectic conditions are required to get the pure bacterial colonies on plate. Bunsen burner is usually used if laminar air flow is not available and tube having the bacteria and loop should be sterile. Three techniques to isolate the bacteria on solid medium are streak plate, pour plate and spread plate.

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