1 (2 pt) Gavin is using a microscope to observe a sample of zooplankton from Lak
ID: 195102 • Letter: 1
Question
1 (2 pt) Gavin is using a microscope to observe a sample of zooplankton from Lake S Describe how he should handle the microscope for the following procedures a. Count the zooplankton under 100x magnification b. Identify the zooplankton under 400x magnification c. Put the microscope away (name at least two important steps to take) 2(1.5 pt). Another lab technician, Malia, is preparing phytoplankton samples for spectrophotometric analysis. Her lab station is equipped with a p1000, p200, and p20. pipette s a. Combine 1 mg of macerated phytoplankton with 500 1 of acetone in a test tube. b. Stir for 2 minutes, then allow the mixture to sit for 10 minutes. Transfer 180 1 of th another test tube and add 96 1 of DI water c. Transfer the resulting mixture from "b" to a microcentrifuge tube she use in each step of the following protocol? 3 (1.5 pt). Malia multiples the above reaction by 10. Write out the new measurements rer hie in proper metric units and determine whether her choice of pipette should change for e 4 (1pt). The phytoplankton that Malia works with is primarily red in color. Malia suspects that phytoplankton produce anthocyanins, a pigment responsible for red coloration in much of the plant kingdom
Explanation / Answer
5. Solution is combination of solute and and solvent. Solvent is generally a liquid. Solvent dissolves solute. Solute is generally a solid. Solute get dissolved into solvent. Example is, sugar may be considered as solute, water may be considered as solvent. Sugar dissolved in water will be known as sugar solution.
6. Unknown molecules here, are solute. When a cell is placed in hypotonic solution(solution having lower concentration of solute as compared to cell) then, water will move from outside to inside of the cell. Reason being, water always moves from hypotonic to hypertonic solution(solution having more concentration of solute). In such situation more water will enter into cell and excess pressure on cell membrane will cause it's rupture.
Same phenomenon is taking place in diagram 2. Example of such phenomenon is when we place a dry grape into water overnight then it gets inflated next morning.
In diagram 1, opposite incidence is taking place. In this diagram solute particles are moving out of cell. That means concentration of solute is more inside the cell. Movement of any molecule takes place from its region of higher concentration to a region of lower concentration. In diagram 1, size of cell is also unchanged. That means same amount of water is also entering into cell.
If a Cell is placed in hypertonic solution, that means concentration of solute is more in surrounding of cell that means water is in excess inside cell. So water will move from cell to its surrounding.
Key concept is, any molecule either water molecule or solute molecule will always go from its higher concentration to its lower concentration.
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