Not sure if my answers are right Microbiology Spring 2018 Skills Assessmell 46 p

Question

Not sure if my answers are right Microbiology Spring 2018 Skills Assessmell 46 points total rowi rfec 5) Below is the 16SRNA gene sequence of E. coli that will be used in PCR. S' aaattgaaga gtttgatost ggeteagctt gaacge ctggc ggcaggccta acacatgcaa 61, gtcgaacggt aacaggaaga agettgetct ttgetgacga gtggaggacg ggtgagtaat actactggaa acggtagcta ataccgcata 8 acgtcgcaag accaaagagg gggacctteg ggcctcttge catcggatgt gcccagatgg 241 gattagctag taggtggggt aacggctcac ctaggcgacg atcectagct ggtetgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg cagcagtggg 361 gaatattgca caatgggcge aagcctgatg cagccatgcc gegtgtatga agaaggcctt 21 egggttgtaa agtactttca geggggagga agggagtaaa gttaatacct ttgctcattg 81 acgttacccg cagaagaagc accggctaac tcegtgccag cagccgeggt aatacggagg uit gtgcaagcgt taatcggaat tactgggcgt aaagcgcacg caggcggttt gttaagtcag 62 atgtgaaate ecegggctca acctgggaac tgcatctgat actggcaagc ttgagtctcg 661 tagaggggg9 tagaattcca ggtgtagegg tgaaatgegt agagatctgg aggaataceg 2 gtggcgaagg eggcccectg gacgaagact gacgctcagg tgcgaaageg tggggagcaa 781 acaggattag ataccctggt agtccacgec gtaaacgatg tcgacttgga 841 ttgaggegtg gcttccggag cta ggttgtgccc acgegtt aagtcgaccg cetggggagt acggccgcaa 901 ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt gaagaacct tacctggtet tgacatccac ggaagttttc agagatgaga 1021 atgtgectte gggaacegtg agacaggtge tgcatggetg tegtcagctc gtgttgtgaa 1081 atgttgggtt aagtcccgca acgagcgcaa cccttatect ttgttgecag eggtcoggcc 1141 gggaactcaa aggagactge cagtgataaa ctggaggaag gtggggatga egtcaagt 1261 cctcgcgaga gcaagcggac c 1201 tcatggccct tacgaccagg gctacacacg tgetacaatg gcgcatacaa agagaagcga tcataaagt gegtcgtagt ccggattgga gtctgcaact 1321 cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg tgaatacgtt 1381 cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1441 gcttaacctt cgggagggeg cttaccactt tgtgattcat gactggggtt aagtgtaac aggtaaceg taggggaacc tgcggttgga tcacctectt a 3 Make sure to write both primers in the 5, 3' direction Forward primer (0.5 points) ALAG Reverse primer (0.5 points) What is the first base that the Taq polymerase inserts AFTER the forward primer? (0.5 points) What is the first base that the Taq polymerase inserts AFTER the reverse primer? (0.5 points)

Explanation / Answer

Forward primer: 5'-AGAGTTTGATCATGGCTCAG-3'
Reverse primer: 5'-GGTTACCTTGTTACGACTT-3'

The first base after the forward primer = C
Complement of C = G
So, the first base to be added by Taq polymerase after the forward primer = C

The first base before the reverse primer = T
Complement of T = A
So, the first base to be added by Taq polymerase before the reverse primer = A

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