How do you calculate the remaining 2 columns? mg protein in tube and mg/ml prote
ID: 194712 • Letter: H
Question
How do you calculate the remaining 2 columns? mg protein in tube and mg/ml protein in tube. This lab was spectrophotometry and the quantitative determination of protein. Weight of egg white was 36.83 grams.
Tube # ml BiuretA540 %T540 Mg protein/tube human ml of stockml of 0.85% saline serum protein (4 mg/ml) 0 0.5 1.0 1.5 2.0 2.0 1.5 1.0 0.5 0 3.0 3.0 3.0 3.0 3.0 0.00 0.11 0.19 0.26 0.37 0 100 77.5 64.5 55.0 42.5 Tube # 1 ml Mg protein |protein in tube in tube A540 % 1 540 mg/ml albumin! globulin| 0.85% |Biuret 0.2 0.4 0 0 0 0 1.0 2.0 saline 1.8 1.6 1.0 0 3.0 3.0 3.0 3.0 0.26 0.44 0.02 0.04 55.0 36.0 95.5 91.5Explanation / Answer
Mg PROTEIN IN TUBE
Tube 6 - 12 mg
Tube 7 - 29.16 mg
Tube 8 - 6.72 mg
Tube 9 - 7.56 mg
Mg/ml PROTEIN IN TUBE
Tube 6 - 1.75 mg/ml
Tube 7 - 4.17 mg/ml
Tube 8 - 1.77 mg/ml
Tube 9 - 0.45 mg/ml
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