1. Only one strand of the double stranded DNA is shown. Is it the coding strand
ID: 194316 • Letter: 1
Question
1. Only one strand of the double stranded DNA is shown. Is it the coding strand or the noncoding strand? Explain how you determined this. What amino acid does the fourth codon of the ORF code for?
2. All Open reading frames in DNA start with an ATG and end with a stop codon. What is the stop codon for this gene? Using the “finder” function in “WORD” determine how many times this three nucleotide stop codon sequence occurs within the DNA sequence shown. Stop codons should only be found at the end of an open reading frame, not within an open reading frame, but you should have found many in this DNA sequence. Explain how this is possible.
Explanation / Answer
ANSWER: DNA is always the coding strand as it contain codons and is complementary to RNA transcripts sequence.
The fourth codon of the ORF codes for the amino acid leucine
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