Which of the following are valid reasons for carrying out enzymatic reactions in

Question

Which of the following are valid reasons for carrying out enzymatic reactions in buffer solutions (Select all that apply.) O Some enzyme-catalyzed reactions produce H ions. The net AG" for most enzyme reactions is a function of solution pH. Some enzyme-catalyzed reactions produce OH ions. O Specifie protonation states of a substrate or substrates in a reaction are often necessary f An error has been detected in your answer, Check for typos, miscalculations etc. before submiting your answer. Submit Answer Try Another Version 1 item attempt remaining

Explanation / Answer

Most of the enzymatic reactions are produce or consume hydrogen ions which can cause to change in pH and can denature the enzyme. As we know that the H+ ions is a positively charged which interact with the negatively charged ions. All enzymes are the proteins and the tertiary structures of the proteins are maintained by the hydrogen and ionic bonds between opposite charge of amino acids. H+ ions has ability to interfere with the hydrogen and ionic bonds of the proteins (enzymes) tertiary structure and alter the enzyme structure (by changing the shape of active site). So pH change can leads to change in active site of the enzyme it also affects the enzymatic reaction because substrate shape is complementary to the shape of the enzyme active site. The enzymatic reaction runs smoothly when enzyme substrate complex can form.

All the enzymes works on the specific range of optimum pH, it is important to maintain the pH of the reaction mixture that’s why buffer solution is used.

Hence, the right answer is option A- Some enzyme catalyzed reactions produce H+ ions.

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