Huang et al. showed several interesting characteristics of HIV-1 RT, but the mos
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Question
Huang et al. showed several interesting characteristics of HIV-1 RT, but the most pronounced effect was in the finger subdomain? Explain what these characteristics are. Huang et al. showed several interesting characteristics of HIV-1 RT, but the most pronounced effect was in the finger subdomain? Explain what these characteristics are. Huang et al. showed several interesting characteristics of HIV-1 RT, but the most pronounced effect was in the finger subdomain? Explain what these characteristics are.Explanation / Answer
HIV-1 RT heterodimer consisting of 2 distinct subunits: p66 and p51.
Both contain 1] thumb 2] palm and 3] fingers with a 4] connecting subdomain.
HIV-1 RT thumb and fingers serve as a clamp aligning template primer of RT correctly toward the polymerase active site.
From the template grip, the active site of p66 contains three catalytic residues which are theorized to aid in the binding of the template primer.This active triad is positioned close to the 3' OH terminus of the DNA primer where DNA polymerization starts.
At the rate limiting step, conformational change where a part of p66 finger subdomain closes down on the incoming dNTP.
Formation of phosphodiester bond between newly incorporated nucleoside and the primer of RT. This generates PPi. Fingers open and PPi to leave the active site.
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