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you are vil held you to calculate the relative size of objects each feld of view

ID: 193695 • Letter: Y

Question

you are vil held you to calculate the relative size of objects each feld of view for your micro feld of view for your microscope. An easy way to determine the size of the is to place a clear plastic ruler (or prepared slide of a section of a ruler) on the stage, much like you slide, and view it through the ocular lenses 2 Using low powes, view the ruler throuph the ocular lenses and estimat 3 Repeat this procedure with the medium-power lens in place 4 Because Obain a side of s section of a raler or a smal, dear plastic raler and place it on the stuge. the size of the field of view by messuring the diameter in millimeters. You should estimate this value to the nearest 0.1 mm. for the field of view the field ofese e of the 40× or 100× lenses. Your instructor can provide the value diameters of those lenses. the ruler (or slide) is too thick to oberve using the higher power lenses, do not attempt to measure Check Your Progress 3.1 The diameter of the field of view of your lowpower lens is 3.2 The diameter of the 3.3 Convert these values to micrometers (um). Remember, there are 1,000 um in 1 mm field of view of your medium-power lens is b Mediumpower lens (10x) = C High-power lens (40x) = d oil-immersion lens (100 x m um (obtain this value from your instructor.) ) 1am (0tain this vatue (rom your instructor.) (obtain 3.4 As a rule, as magnification increases, dlameter of field of view Selre Depth of Field The thickness of an image that is in focus at any point in time is referred to as the depth of field of a lens. Depth of field of your objectives by viewing a slide containing overlapping objects 1 Obtain a slide with a few strands of overlapping colored threads. 2 First view this slide on low power. 3 Conceatrate on a section where the overlapping of the threads can be seen. Are all three colored threads in the magnification of the objective lens in place. You can establish the differences in depth focus using low power? 4 Now switch to medium power and try to determine which thread is on top of the other two. As you focus through the image, some threads will be in focus and others will be blurred. 8 Exercises for the Zoology Laboratory

Explanation / Answer

3.1. diameter of the field of view of lower power lens is 2mm.

3.2. diameter of the field of view of medium power lens is 5mm.

3.3. (a) 2000 micrometers

(b) 5000 micrometers

(c) 20000 micrometers

(d) 50000 micrometers

3.4. decreases