You are studying a strain of yeast that can survive in extreme conditions. You i

Question

You are studying a strain of yeast that can survive in extreme conditions. You identify a copy of the Protein Z gene that is mutated in this strain. In the mutant Protein Z (Z-mut) 12 Gln residues have been mutated to Glu. You need to isolate Protein Z-mut from a yeast strain that expresses both Protein Z and Protein Z-mut. Which protein purification technique would be best to use? Unfortunately you haven't been able to make an antibody that recognizes Protein Z or Protein 2-mut. Molecular exclusion chromatography O lon exchange chromatography O Differential centrifugation O Immunoprecipitation Affinity chromatography What protein detection technique would be best to use to differentiate Protein Z from Protein Z-mut? O SDS-PAGE O ELISA O Enzymatic activity O Isoelectric focusing O Western blot

Explanation / Answer

Ion exchange chromatography will be appropriate. Because mutation results in a difference in charge.

Isoelectric focusing as the PI changed

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