hich of the following experimental techniques can be used to distinguish between
ID: 193578 • Letter: H
Question
hich of the following experimental techniques can be used to distinguish between various topoisomers of DNA?
Question 10 options:
DNA sequencing
Gel Electrophoresis
NMR
X-ray crystallography
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Question 11 (1 point)
Bacterial infection of the urinary tract can be treated with nalidixic acid. The action of this drug is to inhibit
Question 11 options:
Primase.
DNA helicase.
Telomerase
DNA topoisomerase.
DNA ligase.
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Question 12 (1 point)
Which of the following is an accurate statement regarding topoisomerase I?
Question 12 options:
Type I topoisomerases catalyze the relaxation of supercoiled DNA.
Type I topoisomerases catalyze the separation of DNA double helices into single strands.
Type I topoisomerases catalyze cleavage of both DNA strands and their resealing.
Type I topoisomerases couple ATP hydrolysis to the removal of negative supercoils from DNA.
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DNA sequencing
Gel Electrophoresis
NMR
X-ray crystallography
Explanation / Answer
ANSWER 10
Gel Electrophoresis is the experimental techniques can be used to distinguish between various topoisomers of DNA. This is because different topoisomers migrate at different speeds across the gel slab owing to their spatial differences.
ANSWER 11
Bacterial infection of the urinary tract can be treated with nalidixic acid. The action of this drug is to inhibit DNA topoisomerase which is how it inhibits their growth and reproduction.
ANSWER 12
Type I topoisomerases catalyze the relaxation of supercoiled DNA
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