eBIT-Mobile 9:21 AM Quiz 2: Attempt 1 Question 3 o out of 1 points This techniqu
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eBIT-Mobile 9:21 AM Quiz 2: Attempt 1 Question 3 o out of 1 points This technique lowers gene expression by targeting mRNA with a silencing complex that can cleave mRNA Question 4 out of 1 points "If you were to perform a 2D gel and mass- spectrometry experiment, comparing the proteins present in a population of cells before and after addition of a drug, which of the following would you expect to be true?" Question 5 out of 1 points Which of the following is true regarding CRISPR/Cas technology? Question 6 1 out of 1 points A researcher wants to identify genesExplanation / Answer
3) This technique lowers gene expression by targeting mRNA with silencing complex that can cleave mRNA:
siRNA-mediated target mRNA cleavage: siRNAs repress gene expression by the cleaving target mRNAs and leading to a sequential degradation through RNA-induced silencing complex (RISC). The siRNA cleavage site on the mRNA target is normally located in the middle of the region spanned the siRNA:target duplex.
5) Which of the following is true regarding CRISPR/Cas technology?
CRISPR/Cas technology is used to edit parts of genome by removing, adding or altering the sections of DNA sequence. This technique consists of two key molecules to introduce a change into the DNA. These two molecules are: an enzyme called Cas9 and a piece of RNA called as guide RNA. The guide RNA is designed to find and bind to a specific sequence in the DNA. Cas9 follows the guide RNA and makes a cut across both strands of the DNA.
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