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Questions 1 Resolving power (R.P) is a function of the wavelength of light used

ID: 193320 • Letter: Q

Question

Questions 1 Resolving power (R.P) is a function of the wavelength of light used and the numerical aperture (N.A.) of the objective lens. It is expressed as the smallest distance between two points that can still be discerned as discrete entities. Given that R.P = wavelength / (2 x N.A.) calculate the resolving power of your microscope when using the oil immersion lens. Assume that visible light has an average wavelength of 0.55 um (a = 0.000001 meters). Rlut light has the shortest wavelength in the visible spectrum. If blue light were used with a microscope resalitkn

Explanation / Answer

Wave-length = 0.55 m and typical value of NA of the lens = 1.1

Resolving power RP = Wave-length / (2 x NA) = 0.55 m / (2 x 1.1) = 0.25 m.

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