Tube 84: B. EFFECT Of TEMPERATURE on ENZYME ACTIVITY b as prepared a erude extra

Question

Tube 84: B. EFFECT Of TEMPERATURE on ENZYME ACTIVITY b as prepared a erude extract of the enayme catalase from potatoes (otber vegetabie at which for catalase from for catalase can sources could also be tested). Each enzyme has an optimal temperature This is the termperature it shows maximal activity. In this section you will determine the optimal temperatur be different. MPORTANT: Follow Table 4-2and the instructions below for the experimental 1. Use tubes #1 -85 in the test tube rack; make sure the tubes are clean before you begin. 2. enryme source (plant, animal, etc.), the optimal conditions g on the Into tubes #1-84, pipet 2 mL of "Potato Extract" and 2 mL of distilled water (dH,O) Into tube #5, add 2mL ofthe boiled-Potato Extract" which has been prepared for you distilled water 3. ad 2 mL of 4. Set up the five 50 mL tubes as follows: Tube # 1: 0°C-set on ice Tube #2: 10°C set in waterbath Tube #3: Room Temperature (-250-remains in rack at your table Tube #4:37°C-set in the waterbath Tube #5: 100°C (use the boiled "Potato Extract", remains in rack at your table) Table 4-2. Experimental set-up for the effects of temperature on catalase activity Tube# t Temperature Extract" dHO% of sample 2 mL 2 mL 2 mL 2 mL 2 mL boiled Ice: 0°C 10°C 2 mL 4 mL 4 mL 4 mL mL 2 0 2 mL 37 C 100°C 2 mL "Potato Extract" 30

Explanation / Answer

The temperature values like 0, 10, 25, 37 and 100 degree should be plotted in x-axis.

Rate of reaction shouldbe plotted in y-axis.

After performing the experiment, respective values are plotted in the graph, the temperature at which degree the enzyme catalase is higher is identified.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.