True or False? After using cloning techniques you want to verify you have insert

Question

True or False?

After using cloning techniques you want to verify you have inserted the correct gene of interest into the vector. To verify if your recombinant plasmids contain the gene of interest you split your sample into three tubes each containing restriction enzyme(s) (EcoRI, Hindlll, EcoR1 and Hindlll respectively). According to the plasmid map shown this is how the agarose gel should look after electrophoresi Haelll onTA Hind Kpnl IRS EcoRI 17 16 15 Hind pSKU146 14,615 bp 10 ICEF-like 8.0 Kb Hinf Acal

Explanation / Answer

EcoR1 will linearize the vector since only one cutting site is there so single band in lane one is correct.

HindIII will cut the vector at 2 sites so 2 band on the gel is correct.

Combination of it will give 3 band on the gel so above mentioned question and there explanation is correct.

Or depiction is true.

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