54. The substitution of a G for an A in the protein coding sequence of BRCAT gen

Question

54. The substitution of a G for an A in the protein coding sequence of BRCAT gene introduced a SNP. This SNP changed the amino acid incorporated into the BRCA1 protein, resulting in the disease symptoms of breast cancer. Unaffected individuals have the DNA sequence AAGCTT, a Hind restriction enzyme cut site, in the BRCA1 gene. The G substitution (AGGCTT) eliminates this restriction enzyme site A. Explain how you would use this information to design a screening test for breast cancer. Write out all of the major steps to do this genetic test, starting with getting a sample of cells from the patient (tell me where you would get the cells). Be specific in using information given in the paragraph above when telling me about your plan. (7 pts) HINT: USE YOUR MODULE 3 LAB TO HELP YOU Step 1: Obtain cells from patient from their Step 2: Step 3: Step 4 Step 5:

Explanation / Answer

Step 1 : obtain cells from patient from their breast cancerous tissue.

Step 2 : Culture the cancerous cells and isolate the DNA from those cells.

Step 3 : PCR amplification of BRCA1 gene using specific primers.

Step 4 : RFLP of the amplified gene products using the restriction enzyme Hind III,BRCA1 gene will not be digested

Step 5 : Screening of the cancerous cells using their restriction profile.

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