• Prokaryotic Initiation of Replication: formation of replication forks and star
ID: 193114 • Letter: #
Question
• Prokaryotic Initiation of Replication: formation of replication forks and start of polymerization
o What are the names of the binding sites contained in the replication origin and what proteins do they bind?
o What role does the enzyme Dam methylase play in the initiating replication?
o Describe the roles of DnaA, DnaB, DnaC•ATP, and Hu proteins in the initiation of prokaryotic replication.
o What is the DUE region in the replication origin and what role does it play in initiation of prokaryotic replication?
o Is primase (DnaG) required for the initiation of replication?
What role does primase play in replication? Is that role necessary for replication to begin?
o What is the function of DNA Gyrase?
o Put all of the above together and tell the story of the initiation of prokaryotic replication.
• Describe the function of ORC, Cdc/Cdt complex, MCM, and Cdc45 protein complexes in the initiation of eukaryotic replication.
• Elongation of the DNA polymer: prokaryotic replication
o What is the primary function of DnaG during elongation? Why is this necessary?
o Tell the story of the actions of DNA Pol I and III during elongation.
o What is the function of single strand binding proteins (SSB)?
Are SSB found primarily on the leading or lagging strand?
o Where is helicase found in the replisome?
o Does the subunit of DNA Pol III remain bound to both strands of DNA during replication? Why?
Explanation / Answer
DnaA helps to unwound the ori and thus replication to proceed.
DnaB-opens the replication fork during replication, it is a helicase.
DnaC.ATP-it is the loading factor of helicase DnaB
Hu protein-resembles the eukaryotic Histone H2B, inser negative supercoiling along with the topoisomers
DUE (DNA unwinding element)region- it is the region where initiation of DNA unwinding happens at the ori.
DnaG- without it DNA replication won't happen.DNA polymerase requires a 2'OH for initiating the olymerisation reaction.dnaG are the DNA primase which add short RNA strands (which has 2'OH).
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.