one of your classmates is still unsure as to how transformation works. so, he pe

Question

one of your classmates is still unsure as to how transformation works. so, he performs the following experiment:- A competent E. coli culture is tranformed with DNA coding for penicillnase ( and enzyme that degrades the antibiotic penicillin) . - A different sample of the same strain of E. coli is also transformed with DNA coding for penicillinase. in this case, though , a small amount of penicillin ( the antibiotic) is also added to the reaction tube during the transformation process. what results would you expect?
a) neither organism would be transformed because you cannot put a gene for antibiotic resistance into a prokaryote
b) the tube without penicillin would have many more transformed organisms
c) the two tubes would produce very similar results
d) the tube with penicillin would have significantly fewer transformed organisms

Explanation / Answer

Ans. the tube without penicillin would have many more transformed organisms,

and the tube with penicillin would have significantly fewer transformed organisms are the expected results.

Reason: If the antibiotic is added in the transformation reaction, then the cells will die, as the cells are not undergone transformation. And cells without transformation with the gene encoding antibiotic resistance will be susceptible to the penicillin.

In this way the cells that remain alive due to incorporation of penicillinase gene, will becomes penicillin resistant by degrading it. So the tube without penicillin will have more transformed cells compared to the tube with penicillin in reaction.

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