1. (2 each) Define/Compare/Contrast/Distinguish these three terms F plasmid F\'
ID: 192823 • Letter: 1
Question
1. (2 each) Define/Compare/Contrast/Distinguish these three terms F plasmid F' plasmid (and how is it formed) Hfr cell (and how is it formed) 2. (2) With the technique of interrupted mating, four Hfr strains were tested for which order they transferred genes to an F strain. Here are the results: (Note the order transferred in each case is top to bottom. The top gene was transferred first, and the bottom gene transferred last.) strain I strain 2 strain 3 strain4 strain5 A ZZC B L E E PC Z N R A L Draw a circular map of the chromosome, showing the order of the genes 3.(2) In 3 transduction experiments, here are the donor cell and the recipient cell (each row of the table is a separate expt). For each row, you select for a marker as shown (remember that select means to kill off everything except the cells that have that marker) and among those that live, you ask if they contain or do not contain the unselected marker. The last column shows the % of cells that contain the unselected marker. From these data, figure out the order of the genes and which two are closest together. Hint: there are only 3 possible orders. Donor Recipient Selected marker unselected marker% Et GtegEt the g gene the g gene the e gene 26% 54% 5%Explanation / Answer
1)F plasmid: are those that have F factor in them. This allows the bacteria to transfer genes from one to another which lacks the factor by means of conjugation
F' plasmids are formed due to incorrect excision from the chromosomes as a result,along with the F palsmid there will be host bacterial DNA sequence.
Hfr has entire f factor incorporated into the main chromososme.
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