The technique used here was double immunofluorescence. Which of the following be

Question

The technique used here was double immunofluorescence. Which of the following best describes how this double staining is achieved? Choose three answers.

Each primary antibody is labeled with a different fluorophore – red or green
The primary antibodies used are made in different species and recognize different antigens.
The primary antibodies are made in the same animal species, but against different antigens (i.e. both are rabbit antibodies, but one recognizes K14 and another recognizes K1)
The secondary antibodies are labeled with a different fluorophore – red or green
The secondary antibodies are made in the same animal species, but recognize different primary antibody species (i.e. both are goat antibodies raised against either rabbit or mouse FC regions)

Explanation / Answer

The correct option are

1) the secondary antibodies are made up of animsl species but recognize diffdiffe primary antibody..

2) each primary antibodies is labeled with different flouropore

3) the primary antibodies are made up of same animal species different animal species..

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