7. (4 pts) All the amino acids found naturally in proteins are chiral, with one

Question

7. (4 pts) All the amino acids found naturally in proteins are chiral, with one exception a. Name the exception This amino acid is not chiral because its alpha carbon has two nitrogen /hydrogen/oxygen atoms attached directly to it. (Circle the correct choice.) b. 8. (2 pts) The isoelectric point (pl) of an amino acid, peptide, or polypeptide is the pH at which: a. b. c. d. e. the molecule has no electrically charged functional groups in it. the molecule has an overall positive charge. the molecule has an overall negative charge. the number of positive charges in the molecule is equal to the number of negative charges. the number of positive charges in the molecule is twice the number of negative charges. 9. (8 pts) You learned about three chromatographic methods for protein purification: size-exclusion chromatography, ion-exchange chromatography, and affinity chromatography. Which one of these methods would you be MOST likely to use first if you were trying to purify: a. b. c. d. A protein that binds strongly to the sugar maltose A protein with a very low molecular weight (

Explanation / Answer

7. a. Glycine

b. Hydrogen

8. The molecule has no electrically charged functional groups in it.

9. a. Affinity chromatography

b. Size exclusion chromatography

c. Affinity chromatography

d. Ion exchange chromatography

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