Name: Section #: 8. Complete the following table comparing and contrasting DNA r

Question

Name: Section #: 8. Complete the following table comparing and contrasting DNA replication and transcription? DNA Replieation Characteristic How many strands of DNA are used as the template? Direction of synthesis of the new strand? Transcription Direction of movement of polymerase along the template strand? What is the "end product" of this process? What happens to the new strand as it is being made? Where does this process occur? Enzyme(s) involved in the process and their specifie role. Briefly list the specific steps to complete the process. Be use to include the enzymes you listed above and outline in locture. 9. What process is shown in the diagram? DNA Replication or Transcription? How do you know?

Explanation / Answer

how many stands of DNA are used as template-

DNA replication- both of the strands are used in replication. so 2. transcription- only on of the strand is used as template.

Direction of synthesis of newly strand?

DNA replication- 5' to 3'

Transcription- 5' to 3'

Direction of movement of polymerase along the template?

DNA replication - 3' to 5'

Transcription- 3' to 5'

What is the end prouct of this process?

DNA replication - DNA

Transcription- RNA

What happens to the newly strand as it is being made?

DNA replication- newly strand form double helix with the template.

Transcription- Post transcriptional modification.

Where does this process occur?

DNA replication- nucleus

Transcription- nucleus

Enzyme involve in the process and their role?

DNA replication-

Helicase- unwind the double helix of DNA

DNA primase- copies the DNA strand to short RNA complementary strand

Topoisomerase- relieve the negatie supercoiling of DNA

SSB protein- single strand binding protein that avoid the formation of double helical strand

DNA polymerase III- elongate the replication in 5'-3'

DNA polymerase I- remove the RNA primer after elongation.

DNA ligase- join the nick form during replication.

Transcription-

Transcription factors- recognises the promoter sites and help binding of RNA polymerasa to the DNA strand.

RNA polymerase- elongate the synthesis of RNA from 5'-3'

Rho- terminate the RNA synthesis by destabilizing the complex between mRNA and template.

Briefly Listed the specific steps:

DNA replication-   Initiation (Helicase, topoisomerase, DNA primase), elongation ( DNA polemeraseIII, SSBP) , termination ( DNA ligase)

transcription- Initiation (transcription factors), elongation ( RNA polymerase) and termination( Rho factor).

9. In the diagram shown there is only one strand on product has been synthesized and its is transported to cytoplasm from nucleus. So, the process in transcription.

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