0 Question 2. (9 pts) You are trying to separate the four peptides: ENEEDDRYSH =

Question

0 Question 2. (9 pts) You are trying to separate the four peptides: ENEEDDRYSH = molecular weight = 805 g/mol GGIANTICCC molecular weight = 748 g/mol ARRY LKKAN = molecular weight = 1109 g/mol PLYAPLA-molecular weight = 976 g/mol Which peptide would elute first (run through the column fastest) using a size exclusion column?-ARR9LICCAN Which peptide would bind most tightly to a thiol-capture column (which forms covalent bonds to molecules containing sulfhydryl groups)?_ GGLANTISss Which peptide would elute last from an ion exchange resin with positively charged beads when the experi ph 72 (Hint: which one would stick the most to the positively charged beads) ENEE

Explanation / Answer

The first Peptide ENEEDDRYSH Has rich in aspartic acid and glutamic acid ( D and E respectively) so they have a free " COOH ----" side chain . And in neutral pH the "COOH --- " chain will appear as " COO-" so the peptide will have nagatively charged side chain which will stick to a positively charged beads and elute at last.

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