6. You are given a tube of saliva that has already been centrifuged. You also ha

Question

6. You are given a tube of saliva that has already been centrifuged. You also have the following items: test that hold 6 ml, a dispensette that dispenses 4.5 ml of a phosphate buffer, and a P1000 micropipette. volumes of saliva (or diluted saliva) and phosphate buffer that should be used to prepare the fo dilution 1/ 10 saliva solution = 1/1 00 saliva solution = 1 / 1,000 saliva solutions 1/10,000 saliva solution =- . 1/100,000 saliva solutions ml of purified saliva + ml phosphate buffer ml of 1/ 10 saliva + ml phosphate buffer ml of 1/100 saliva + ml phosphate buffer ml of 1/1,000 saliva + ml phosphate buffer ml of 1/10,000 saliva +- ml phosphate buffer

Explanation / Answer

1/10 dilution = 1 mL of purified saliva + 9 mL of phosphate buffer
1/100 dilution = 1 mL of 1/10 saliva + 9 mL of phosphate buffer
1/1000 dilution = 1 mL of 1/100 saliva + 9 mL of phosphate buffer
1/10000 dilution = 1 mL of 1/1000 saliva + 9 mL of phosphate buffer
1/100000 dilution = 1 mL of 1/10000 saliva + 9 mL of phosphate buffer

In each step, 1/10 dilution is increased.

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