cukaryote. Your PI now asks You look at your cells and use the eel\'s nucleus is
ID: 191258 • Letter: C
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cukaryote. Your PI now asks You look at your cells and use the eel's nucleus is 192um (as calculated above-see Figures 4.2- a s measurement seems too large, so before reporting it you double check ur Work and realized that you were using the 40x objective to view your cells, not the 10x objective. 2) Recalculate the size of the nucleus of the cell shown in Figure 4.3 using the 40x objective picture in Figure 4.2. Postlab: 1) Explain why you had to do multiple size measurements of each cell ype to determine an average size. 2) Which of the cell types you observed was the largest? How did you determine this? 3) In this lab you looked at both unstained and stained cells, what structures were revealed in the stained samples that were not easily visible in the unstained samples? 4) What cellular structures were stained in the onion skin when you stained with safranin O? 5) What are two differences you were able to observe between the mammalian cells (cheek cells) and plant cells (onion skin)?Explanation / Answer
From the figure at 40X magnification, it is evident that 3.5 ocular divisions coincide with 0.5 divisions of the stage micrometer.
Thus, the calibration factor or least count of ocular micrometer= (Stage micrometer divisions subtended by ocular micrometer X Value of one stage micrometer division in µm)/ Ocular micrometer division subtended by stage micrometer
=(0.5 x 10)/3.5
=1.45 µm
Now, diameter or size of the cell at 40X magnification= No.ocular divisions occupied by the nucleus X calibration factor
=1.6 x 1.45
=2.32µm
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