In a recombinant DNA cloning experiment, how can we determine whether DNA fragme

ID: 190644 • Letter: I

Question

In a recombinant DNA cloning experiment, how can we determine whether DNA fragments of interest have been incorporated into plasmids and, once host cells are transformed, which cells contain recombinant DNA? Select all that apply The bacterial host chromosome will increase in size because it will incorporate the plasmid that was inserted When DNA fragments of interest have been incorporated into a plasmid, the result is a change in the function of a gene or genes in the plasmid. Bacteria may lose resistance to certain antibiotics depending on the location of the DNA insert. In the presence of X-gal, medium-driven color change is observed in bacteria that contain a recombinant plasmid. O Submit Request Answer

Explanation / Answer

Ans A) For this question option C & D.

Explain:- The incorporation of the fragment DNA of interest into the plasmid in the transformed host cells.makes the host cell resistance in response to some certain antibiotics. When the normal host with out recombinant DNA and cell with recombinant DNA is placed in certain resistance medium then the host with recombinant DNA survives. This indicate the incorporation of the interest DNA into the host cell.

Blue and white screening is a process through which we confirm the pressence of recombinant DNA in the host cell.

Ans 2) labelling of the particular gene and then PCR.

Ans 3) Option B, C & D.

PCR occur by the denaturation of the dsDNA into ssDNA. the primer bind to one of the ssDNA to bind the DNA polymerase. After the activity of the DNA polymerase, the DNA reanneal at the low temperature to form dsDNA.. Then another round of the cycle takes place in which the dsDNA is denatured at high temperature to form ssDNA.

Thenm again the primer will bind alond with DNA polymerase and annealind to form dsDNA.

Ans4)

Next generation sequencing.

Deoxytidenucleotide chain termination sequencing.

read lenght upto 1000. capillary electrophoresis methord

Read sequence 200- 400bp.

A solid phase.

a series of chemiluminescent reaction.

squencing by synthesis

Pyrosequencing

1st generation sequencing