#3: Enzyme reaction catalysis For each of the diagrams below, indicate which enz
ID: 190351 • Letter: #
Question
#3: Enzyme reaction catalysis For each of the diagrams below, indicate which enzyme catalyzes which reaction step by placing the number of the enzyme (from the list provided) in the proper position on the reaction sequence provided A. Step-wise reduction of chlorinated ethene compounds by reduction (removal of a single chlorine atom) is accomplished by the following enzymes in Dehalococcoides: 1. Vinyl chloride reductase 2. Trichloroethene reductase 3. Dichloroethene reductase Reaction Sequence: H 2H 26 H C=C C=C CI B. Enzymes that break down starch molecules into simpler sugars (disaccharides and monosaccharides) work in a variety of ways on one form of starch known as amylose. One such action occurs by the first enzyme cleaving off disaccharides at the end of the chain, and then a subsequent enzyme breaks each disaccharide into monosaccharides (single-ring sugars) 1. Maltose hydrolase 2. Beta (B)-amylase Reaction Sequence: Amylose > Maltose glucoseExplanation / Answer
A) The above reaction should takes place in stepwise reduction with the help of the following enzymes
1) In first step reduction Trichloroethene reductase is used
2) In second step dichloroethene reductase is used
3) In third step vinyl chloride reductase is used
B) 1. Enzyme Maltase breaksdown maltose into glucose. Maltose is a disaccharide which is obtained after starch is hydrolysed.
2. Enzyme beta amylase attacks 1:4 linkage of amylose which is the constituent of starch to form disaccharide maltose.
The step number 2 always takes place first than that of step number 1 in case of step wise breakdown of starch
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