-Articulate and apply what the parameters Km, Vmax tell us about an enzyme and i
ID: 190329 • Letter: #
Question
-Articulate and apply what the parameters Km, Vmax tell us about an enzyme and its properties.
-Draw and interpret Michaelis-Menten graphs
-What can you say about Km and the reaction velocity in this plot of an enzyme (ENZ3) and its mutated form (ENZ 1)
-What happens to Vmax if the amount of the enzyme added is doubled?
Why: Iftwo enzymes, in different pathways, compete for the same substrate, then knowing the values of Km and Vmax for both enzymes permits prediction of the metabolic fate of the substrate and the relative amount that will flow through each pathway under various conditions. Objectives: o Articulate and apply what the parameters Km, Vmax tell us about an enzyme and its properties. o Draw and interpret Michaelis-Menten graphs What can you say about Km and the reaction velocity in this plot of an enzyme (ENZ 3) and its mutated form (Enz 1) 0.T5 0.5 0 50 100 150 200 250Explanation / Answer
Answer 1.
Km is that substrate concentration at which Vmax is half. According to the given plot, Em1 has a Vmax of 0.9 and Em3 has a Vmax of 0.75.
Vmax is half at 0.45; at this value of Vmax the Km comes out to be: Km(Em1) = 10mM
Vmax is half at 0.375; at this value of Vmax the Km comes out to be: Km(Em3) = 10mM
So, you can say about the Km that it is equal for both; Vmax is greater for Em1 than Em3.
Note: Units not given; I’m assuming the units myself.
Answer 2.
According to Michaelis Menton equation:
Km = Vmax [S]/Km+[S]
Note that value of Km and Vmax depends on substrate concentration, and not on the enzyme concentration. So, there will be no effect even if you double the enzyme concentration.
Answer 3.
High Km value means enzyme has low affinity for the substrate and vice versa.
Similarly high Vmax means that the enzyme has high affinity for the substrate.
Answer 4.
Michelis- Menton plot is already drawn.
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