If enzyme concentration doubles, how is Km affected? The Km change is different

Question

If enzyme concentration doubles, how is Km affected?

The Km change is different for each enzyme

Which of the following is true for an enzyme with a high Km?

For an enzyme with a Km of 10 uM, how does the initial reaction velocity change if the substrate level is raised from 1 uM to 2 uM?

What is TRUE about competitive and non-competitive inhibitors?

If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present, which of the following would have the least effect on the rate of reaction?

Add a moderate amount of an irreversible inhibitor

Decrease the Temperature of the Reaction by a moderate amount

How can Vmax be approached despite the presence of a competitive inhibitor?

Explanation / Answer

(1) Km remains the same

Km does not depend on enzyme concentration, therefore If enzyme concentration doubles, Km remains unaffected

(2)The enzyme will have a low affinity for substrate and require high amounts of substrate to reach Vmax

Km is a measure of affinity of an enzyme with the substrate

(3)The initial reaction velocity nearly doubles

Increasing Substrate Concentration increases the rate of reaction

(4)Both types of inhibitors can be reversible

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