While working with a highly virulent strain of C. neoformans, you isolate a muta
ID: 190143 • Letter: W
Question
While working with a highly virulent strain of C. neoformans, you isolate a mutant deficient in the production of the pigment melanin (the pigment that gives our hair and skin color). Virulent C. neoformans forms black colonies, while the mutant colonies are white. Describe an experiment (in detail) you could perform to determine if virulence is due to pigment production
This might be easy and I could be overthinking it, but I was thinking to make one plate of the mutant, then one plate of the C. neoformans then plating a colony of C. neoformans into the mutant plate to see if it would cause the mutant to form black colonies. I don't know if that's right protocol?
Explanation / Answer
ANSWER: FIRSTLY , we need to isolate the strains of C. neoformans and also the mtant lines as well.
then, yes the protocol as thought will be correct to move ahead by growing one plate of the mutant, then one plate of the C. neoformans then plating a colony of C. neoformans into the mutant plate to see if it would cause the mutant to form black colonies. Then we need to isolate both the strains by adding melanin pigment in both, in wild type we will see good production of melanin pigment production but in mutant lines we will see no such melanin production.
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