5) Using the Fnu4HI CAPS assay, you genotype yourselfor at TAS2R38 and learn tha

Question

5) Using the Fnu4HI CAPS assay, you genotype yourselfor at TAS2R38 and learn that you are homozygous for the AVI/AVI allele. Next, you decide to genotype a sample of DNA extracted from a Neanderthal bone You do not wear gloves while setting up the PCR reaction. You do not perform a negative control reaction. You digest the PCR reaction with Fnu4HI and obtain the result shown below. What can you conclude about the genotype of the Neanderthal (AVI/AVI, AVI/PAV, PAVIPAV)? Explain your answer. 300b 200b 100b 50bp Lane 1, 100-bp ladder. Lane 2, Neanderthal sample.

Explanation / Answer

AcclAccor to the information, the target genotype for this study is AVI. Here, it must be clearly noted that when the genotype is analysed using gel resolution, each band correspomds to an allelic form. Thus, presence of a single band suggests homozygosity for any allele type. However, presence of two bands is a definite suggestive that the sample is heterozygous for the allele, i.e. AVI/PAV.

The possibility for this observation can be contamination from human sample since the experimenter did not wear gloves during the procedure.

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