HELP! ASAP Based on article https://www.ncbi.nlm.nih.gov/pubmed/21282636 This is

Question

HELP! ASAP Based on article https://www.ncbi.nlm.nih.gov/pubmed/21282636 This is the URL to find the article for free and the only way to upload.

Be detailed, please. Read the article to answer questions below that is all

(microbial symbiosis)

a. identify and explain the research question and hypothesis.

b what is the most confusing concept explained and why? What should be a future experiment to answer some important questions.

c Pick 2 figures, or specific figure panels most effective in explaining the results of the research and explain why you chose them Describe the methodology behind each and briefly summarize the results. d. What were the main conclusions based on b and c?

Explanation / Answer

Hypothesis: Normal gut microbiota colonization by influence brain development of mammals and adult behavior. Process of microbial colonization starts mechanisms of signaling that alter neuronal circuits involved in control of motor as well as anxiety behavior. gut microbiota could regulate the adreno-corticotrophic hormone (ACTH) level in young mice. microbiota of gut also regulate synaptophysin as well as PSD-95 both in the striatum during synaptogenesis sensitive period. exposure to metabolites of gut microbiota i.e. during embryogenesis the pregnant mother flora originate, brain development could also be affected.

c) Germ Free (GF) Mice shows enhanced Motor Activity as well as decreased Anxiety-Like Behavior: in figure1 GF mice shows enhanced motor activity. In this experiment set, noth adult germ free (GF) as well as specific pathogen free (SPF) mice subjected with a normal microbiota of gut to tests for analytic activity and anxiety. GF and SPF mice were placed in an open-field box. Their motor activities like locomotor and rearing spontaneous motor activity, were recorded for 60 min. GF mice display more distance traveled also greater open field center exploration (P < 0.05; Fig. 1A). GF also show greater rearing activity as compared with SPF (GF vs. SPF, 489 ± 43 vs. 369 ± 50, P = 0.088). During the initial exposure to open field , GF and SPF mice both showed identical locomotor activity (Fig. 1B) showing that in GF mice, the enhanced locomotor activity was not initiated by originality. Instead, significant differences between groups were detected in habituation over time (repeated measures ANOVA, main effect F(1, 70)= 6.28, P < 0.05). Thus, longer distance tavelled by GF mice significantly (Fig. 1 B and C) and during the interval of 20- 60 min testing, they significantly spent (P < 0.05) more time in fast and slow locomotion both (Fig. 1D). In fig 2, GF mice shows reduced anxiety-like behavior. this 2 figure better explain the hypothesis.

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.