BIOL 151- Homework 3, Deciphering the DNA Code Complete this homework and upload
ID: 189302 • Letter: B
Question
BIOL 151- Homework 3, Deciphering the DNA Code Complete this homework and upload it to Blackboard as a single Word or PDF file. Do NOT upload multiple jps files. If you are having trouble, ask for Blackboard help at ITS 1. Shown below is the sequence from human chromosome 1 that contains a small gene: 5- TGTCGCGCGCGGATATGAGACCCCTACGGGATATATTAGTTTACGCCCGGCG-3 3 - ACAGCGCGCGCCTATACTCTGGGGATGCCCTATATAATCAAATGCGGGCCGC-5 S-CGCGCGCGG-3' 3'-GCGCGCGCC-5 The Promoter is: The Terminator is: 5'-GCCC-3 3'-CGGG-5 Introns: mRNA sequence 5'-CCCC-3' and 5'-AUAUAU-3 a. In the space provided above, write the sequence of nucleotides in the pre-mRNA that would be formed from this piece of DNA. Assume that the transcribed gene starts immediately after the promoter and that transcription proceeds from left to right. Be sure to indicate the 5' and 3 ends of your mRNA. b. Now write the structure of the mature mRNA that would be formed. Be sure to indicate the 5 and 3 ends of your mRNA c. Label the start codon and the stop codon on your mature mRNA strand. d. Show the amino acid sequence of the polypeptide produced by this geneExplanation / Answer
Answer:
The pre-mRNA is the Precursor mRNA (pre-mRNA) is an immature single strand of messenger ribonucleic acid (mRNA). A 5' cap is added to the beginning of the RNA transcript, and a 3' poly-A tail is added to the end.
a) pre-mRNA seq is : 5’ AU AUG AGA CCCC UAG GGG AUA UAU UAG UUU AC 3’
A 7-methylguanosine cap is added to the 5 end of the pre-mRNA & A poly (A) tail is added to the 3 end of the pre-mRNA once elongation is complete.
b)
mature m-RNA will not have the intron sequences
so it will be : mRNA seq is : 5’ AU AUG AGA UAG GGG UAG UUU AC 3’
c)
The start codon is :
in bold i.e AUG
5’ AU AUG AGA UAG GGG UAG UUU AC 3’
d)
The protein/ peptide will be
Met- Arg-Tyr-Gly
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