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2. How does the Winogradsky column differ from Koch\'s plate media? a. Koch\'s m

ID: 189177 • Letter: 2

Question

2. How does the Winogradsky column differ from Koch's plate media? a. Koch's media creates a gradient from oxygen-rich conditions at the surface to highly reduced conditions below. b. The bacteria that Winogradsky isolated can grow only on inorganic minerals. c. The Winogradsky column is used for growing extremophiles. d. The Winogradsky column uses the kinds of nutrients that feed humans. 3. Suppose Pasteur's swan-necked flasks containing boiled broth became cloudy (evidence of bacterial growth) twenty-four hours after boiling. Which choice could best explain the turbidity or cloudiness in the broth without supporting spontaneous generation? a. Endospores in the broth survived boiling and grew after the broth cooled. b. Contaminating organisms in the broth killed by boiling became alive again after the broth cooled. c. Chemicals in the broth came together to form living organisms. d. The broth allowed light to pass through it with less interference after boiling. 4. The word "vaccination" is derived from the Latin word '"vacca", which means a. inject b. smallpox c. immunize d. cow e. pustule

Explanation / Answer

For question 2:

The correct option is B.

The winogradsky column is basically a glass with soil, water and an indicator. It is used for the growth of soil organisms. Precisely the water that is added allows that there is little oxygen in the bottom of the column and in the upper part more oxygen. In addition to favoring some microbial populations sources of inorganic carbon or organic carbon of difficult degradation (such as soap) are placed

For question 3

The correct option is A.

Some bacteria have resistance structures called endospores and these can easily withstand the boiling temperature for a short period of time. A process that ensures the elimination of the endospores is called "Tindallization" in which the broth is boiled, it is left to cool, it is placed at incubation temperature for one hour (in such a way that the bacteria think that they are in safe conditions and begin to grow ) and after this time it comes back to boil (to kill bacteria that thought they were in safe conditions). The boiling-cooling-incubation process is done in triplicate to ensure there are no spores.

For question 4

the correct answer is D

It is in honor of the cows, who invented the vaccine realized that people who were immunized worked with cows they were the ones who milked the cows to obtain the milk.

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