Given that you set up your PCR reactions correctly, would you expect to see a ba

Question

Given that you set up your PCR reactions correctly, would you expect to see a band for GMO positive control (plant) template DNA amplified with PSII primers and with GM primers? Choose the single, best answer:

PSII yes, GM yes

PSII no, GM no

PSII no, GM yes

PSII yes, GM no

A.

PSII yes, GM yes

B.

PSII no, GM no

C.

PSII no, GM yes

D.

PSII yes, GM no

QUESTION 1 Why is non-GMO (negative control) necessary for this experiment? Choose all that apply. OA We didn't really need this control B.We need to ensure that PCR should not work for non-GM foods We need a known non-GM sample to compare our test samples to DWe need to verify that GM primers are specific, and don't amplity other sequences

Explanation / Answer

Answer 1: Choice A (The PSII is a house-keeping gene which is always expressed in the plants, hence used to ensure the purity of the extracted DNA and is always present in the PCR product. Similarly, a non-GMO positive control would be present always in the products since the incorporation of this sequence is made to ensure that positive amplification has taken place and this can be used as a reference standard to investigate the GMO experimental plant)

Answer 2: Choice D (A negative control is incorporated to ensure that the primers so designed in the PCR are very specific for the GMO targets only and do not cross-react unnecessarily with other targets. This ensures the specificity of PCR)

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